The original Star maze was described in the literature as a method to study spatial navigation and its two different orientation systems, allocentric and egocentric navigation. The original maze in the literature utilized a round acrylic arena with a star in the middle. Th MazeEngineers iteration takes the Morris Water maze as an external boundary for the Star Maze Task. Inserts are provided to fit your Morris Water Maze, with the added benefit that the classic Morris Water Maze task can be done in addition to the Star maze. The star is made from Stainless steel for long term use and minimization of corrosion.

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A useful tool in the complex study of spatial navigation in the field of behavioral neuroscience is the web-like design of the Star Maze (Rondi-Reig et al., 2005). The Star Maze, a combination of the widely-used Morris Water Maze (Morris, 1984) and the T-maze (Packard and McGaugh 1996), allows elaborate observation into the complexities of spatial navigation processes, both in the acquisition and organization of spatial knowledge, differentiating among the few possible strategies involved in the task.

The star maze apparatus efficiently integrates inspired components from each design. The rodent is tasked to locate the escape platform submerged under the surface of the water by navigating a five-branch maze, thereby encountering a number of intersections. Similar to traditional maze paradigms, rodents are introduced to the apparatus and are pre-trained, during which rodents swim from a starting position in one alley towards the escape platform located in another alley. During test probes, they are then tasked to locate the same escape platform but from different starting points within the star maze.

Through this process, one may identify the multiple strategies that animals employ in spatial navigation, whether map-based, using a similar sequence of movement, guided by visual intra-maze cues, or a combination of these. The star maze apparatus may, therefore, adopt a variety of experimental protocols (Rondi-Reig et al., 2005), each of which detail the strategy or strategies employed by the rodent.

The star maze can be used as a useful research paradigm in studying and differentiating among the many aspects of spatial learning. The observable behavior of subjects placed within the star maze apparatus lends itself to a more elaborate conception of spatial navigation.

Apparatus and Equipment

The star maze apparatus consists of five alleys forming an inner pentagonal ring, and one alley branching out of each pentagonal junction. The star maze alleys are made up of aluminum for long-term usage. The maze structure is circumscribed by a tub 4, 5, or 6 feet in depth designed to be filled with water made opaque through the usage of pure or powdered milk, white non-toxic watercolor paint, or other such materials. Depending on the chosen experimental protocol, the walls of the star maze apparatus may be fixed with intra-maze cues if the intra-maze cue-based guidance strategy is to be observed. The setup may be placed anywhere in the room or laboratory, and extra-maze (distal) cues may also be maintained.

The apparatus’ design provides the rodent with the task of locating the escape platform hidden in the water, placed in one of the five alleyways. The rodent is pre-trained with one alley as its starting point and tested during probes by letting it start from a different alley. In this manner, different spatial strategies can be identified.

Training Protocol

There are four possible versions of the star maze task. These experimental protocols are briefly described below.

Identifying multiple Strategies: pre-training & probe tests

During the training trials, the rodents are placed in one alley and tasked to look for the escape platform located in another alley within the star maze. Using a standard experimental protocol for identifying multiple strategies (Rondi-Reig et al., 2005; Rondi-Reig et al., 2006), the star maze may adopt a fixed configuration in which the rodent’s starting point during pre-training is alley 1, and the escape platform is fixed in alley 7 (refer to figure below). This particular experimental protocol normally lasts 20 days during which, aside from pre-training, the rodent’s learning ability is also assessed. In this manner, four learning strategies are observed. The map-based or allocentric learning strategy makes use of distal visual cues that are found outside of the maze, typically extra-maze cues fixed within the room or laboratory. The sequential-guidance learning strategy follows a sequence of intra-maze cues found on the walls of the maze. The sequential egocentric strategy, meanwhile, involves learning a sequence of bodily movements, particularly in turning left or right (Rondi-Reig et al., 2005). The rodents’ behavior during pre-training is observed using Noldus Ethovision XT.

Probe tests are usually held in-between pre-trainings, every five days. Rodents are placed in their new starting point in alley 5. A timer is used to measure the mean escape latency. The number of visited alleys and the trajectory employed in locating the escape platform is also recorded through Noldus Ethovision XT. The probe tests last 90 s, after which rodents are placed on the platform if they were unable to do so on their own. The rodent is left on the platform for 30 s.

The probe tests map out three possible routes from a different starting location, alley 5, which allows for valid distinction among the three learning strategies earlier identified: 5-6-7 (allocentric strategy), 5-6-8-9 (sequential-guidance), and 5-4-2-1 (sequential-egocentric). These probe tests employing the star maze apparatus relies on the strong assumption that rodents placed in different starting points will make use of the same cues employed during pre-training (Rondi-Reig et al., 2005; Rondi-Reig et al., 2006).

Allocentric (map-based) strategy

The allocentric version of the star maze task is designed to solely assess the ability of rodents to organize and map out their spatial knowledge in navigating the 5-alley maze and locating the escape platform. Rodents undergo pre-training, and during allocentric probe tests, they are put in randomly selected starting points for each trial. Latency time, the number of alleys visited, and the trajectory is measured through a timer and Noldus Ethovision XT.

Sequential-guidance and sequential-egocentric strategies

The set-up for sequential strategies does not make use of extra-maze cues. A black curtain is placed around the star maze, and rodents undergo pre-training during which they learn a sequence of intra-maze cues, e.g., white, then black, then chessboard walls, or a sequence of body movements, e.g., left, right, and left, evaluating sequential-guidance and sequential-egocentric strategies, respectively. In the sequential-egocentric set-up, intra-maze cues are eliminated by making maze walls white.

Evaluation of specific brain regions

The complexity of the star maze test has helped detail the specific processes involved in spatial navigation. Advances in research employing the apparatus suggest that different brain regions are involved in the different spatial processes outlined by the star maze test, such as one-body versus two-body turns in egocentric strategies.

Specific experimental deficits, for instance, has helped in observing the involvement of the hippocampus in the spatiotemporal organization of information (Rondi-Reig et al., 2006). These findings may be imperative in the early detection of age-related cognitive deterioration in humans.

Sample Data

The sample data of the star maze allocentric-strategy test is represented by plotting the mean number of alleys visited by rodents across experimental and control groups. The knock-out mice, which had a higher number of alleys visited than control mice, are significantly impaired in accessing memory of the escape platform’s location. These results suggest the involvement of the specific receptor gene taken out of the KO mice in the allocentric or mapping aspect of spatial navigation.

Strengths & Limitations

Sharing similarities with the Morris water maze and the T-maze, the star maze successfully takes inspiration from both tests in offering a detailed analysis of spatial learning capabilities. Rodents’ swimming trajectories are restricted by maze walls, allowing for a more systematic analysis of navigation. The star maze test offers not only a component-based view of spatial processes but evaluates the rodent’s ability to choose and alternate between strategies spontaneously.

Summary and Key Points

  • The star maze apparatus is a combined modification of the Morris water maze and T-maze, designed to observe and identify different strategies in spatial navigation such as map-based and route-based trajectories
  • It allows the observation of a number of spatial strategies as defined by the routes rodents take to locate the escape platform
  • The star maze test has contributed advancements in the field of spatial navigation that have applications in addressing age-related cognitive dysfunctions
  • The maze star apparatus consists of five alleys forming an inner pentagonal ring, and five alleys radiating from the vertices of the inner ring. The structure is circumscribed by a tub, and an escape platform is fixed in one of the five alleys


Morris, R. (1984). Developments of a water-maze procedure for studying spatial learning in the rat. Journal of neuroscience methods, 11(1), 47-60.

Rondi-Reig, L., Petit, G. H., Tobin, C., Tonegawa, S., Mariani, J., & Berthoz, A. (2006). Impaired sequential egocentric and allocentric memories in forebrain-specific–NMDA receptor knock-out mice during a new task dissociating strategies of navigation. Journal of Neuroscience, 26(15), 4071-4081.

Rondi-Reig, L., Petit, G., Arleo1&, A., & Burguière, E. (2005). The starmaze: a new paradigm to characterize multiple spatial navigation strategies. Behavior Research Methods.

Packard MG, McGaugh JL. (1996). Inactivation of hippocampus or caudate nucleus with lidocaine differentially affects expression of place and response learning. Neurobiol Learn Mem 65:65–72.